Part:BBa_K2754000:Design

dCas9 regulated by a TetR inducable promoter

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1832
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 710
  Illegal BamHI site found at 4111
  Illegal XhoI site found at 4841
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Illegal cut sites (EcoRI and XbaI) were removed by site-directed mutagenesis and gibson assembly. Prefix and suffix were inserted by gibson assembly.

Source

Synthesized by Addgene (pdCas9-bacteria, Plasmid #44249)